Department Aquatic Ecology

We chose a Daphnia galeata clone (G100) that was inbred once. Aiming at a high quality assembly, large amounts of DNA were needed, therefore the clone was grown in mass cultures. We extracted DNA of 16033 individuals in 312 extractions. The purity, concentration, and quality of each extract was checked. 445 µg of DNA was obtained.
Sequencing is done using the Illumina platform by the Environmental Genomics, National GeneBank, BGI China using insert libraries of different lengths